Enzyme activity evaluation by differential electrochemical mass spectrometry.
João C P de SouzaWanderson O SilvaFabio H B LimaFrank Nelson CrespilhoPublished in: Chemical communications (Cambridge, England) (2018)
NAD-dependent alcohol dehydrogenase (ADH) enzymes for ethanol oxidation were investigated by differential electrochemical mass spectrometry (DEMS). The broad mass spectra obtained under bioelectrochemical control and with unprecedented accuracy were used to provide new insight into the enzyme kinetics and mechanisms.
Keyphrases
- mass spectrometry
- liquid chromatography
- gold nanoparticles
- molecularly imprinted
- ionic liquid
- capillary electrophoresis
- gas chromatography
- high performance liquid chromatography
- label free
- high resolution
- electron transfer
- hydrogen peroxide
- atomic force microscopy
- alcohol consumption
- density functional theory
- solid phase extraction
- nitric oxide
- clinical evaluation