Isolation of Fluorescent Benzoxazines with Neuroprotective Activity from the Olive Weevil Pimelocerus perforatus .
Aya FujikawaYasuhiro IshiharaEmi OhtaTatsuo NehiraHisashi ÔmuraMylene M UyShinji OhtaPublished in: Journal of natural products (2022)
The weevil Pimelocerus perforatus poses a serious pest problem for olive cultivation in Japan. Two new racemic fluorescent benzoxazines, designated as pimeforazine A ((±)- 1 ) and pimeforazine B ((±)- 2 ), were successfully isolated from P. perforatus . Their structures, including the absolute configurations of their resolved enantiomers, were determined using spectroscopic methods, single-crystal X-ray diffraction, and electronic circular dichroism calculations. The neuroprotective activity of the isolated compounds was evaluated against hydrogen peroxide-induced cellular damage in SH-SY5Y human neuroblastoma cells. Compounds (±)- 1 and (±)- 2 exhibited neuroprotective effects.
Keyphrases
- hydrogen peroxide
- quantum dots
- endothelial cells
- induced apoptosis
- high resolution
- nitric oxide
- living cells
- cerebral ischemia
- high glucose
- cell cycle arrest
- molecular dynamics simulations
- label free
- induced pluripotent stem cells
- magnetic resonance imaging
- drug induced
- magnetic resonance
- fluorescent probe
- dual energy
- cell death
- subarachnoid hemorrhage
- cell proliferation
- pi k akt