First in vivo evaluation of a potential SPECT brain radiotracer for the gonadotropin releasing hormone receptor.
Richard FjellakselAna OteizaMontserrat Martin-ArmasPatrick J RissOle Kristian HjelstuenSamuel KuttnerJørn H HansenRune SundsetPublished in: BMC research notes (2018)
We have used a simple, robust and high-yielding procedure to radiolabel an alpha-halogenated bioactive compound with high radiochemical yield. Literature findings showed similar alpha-halogenated compounds suitable for in vivo evaluations. The compound was found to possess nano molar affinity for the gonadotropin releasing hormone-receptor in a competition dependent inhibition study. Furthermore, liquid chromatography-mass spectrometry analysis in saline, human and rat serum resulted in 46%, 52% and 44% stability after incubation for 1 h respectively. In addition, rat brain single photon emission computed tomography and biodistribution studies gave further insight into the nature of the compound as a radiotracer.
Keyphrases
- mass spectrometry
- liquid chromatography
- pet imaging
- computed tomography
- high resolution mass spectrometry
- endothelial cells
- capillary electrophoresis
- systematic review
- tandem mass spectrometry
- positron emission tomography
- magnetic resonance imaging
- white matter
- minimally invasive
- simultaneous determination
- gas chromatography
- pet ct
- induced pluripotent stem cells
- multiple sclerosis
- risk assessment
- magnetic resonance
- image quality