Assessment of Corneal Endothelium during Continued Organ Culture of Pre-Stripped Human Donor Tissue for DMEK Surgery.
Johannes Menzel-SeveringPeter WalterWolfgang J PlumFriedrich E KruseSabine SallaPublished in: Current eye research (2018)
Corneal EC loss does occur following DM stripping and continued organ culture. EC loss increases with storage past 24 hours, but donor corneas may fall below 2,000 cells/mm2 independently of storage duration. The use of eye bank prepared donor lamellae for Descemet Membrane Endothelial Keratoplasty (DMEK) may increase patient safety by offering standardized quality control before tissue release.
Keyphrases
- patient safety
- quality control
- endothelial cells
- quality improvement
- induced apoptosis
- minimally invasive
- optical coherence tomography
- nitric oxide
- coronary artery bypass
- cell cycle arrest
- type diabetes
- induced pluripotent stem cells
- metabolic syndrome
- acute coronary syndrome
- cell death
- signaling pathway
- cell proliferation
- cataract surgery
- adipose tissue