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CDR grafting and site-directed mutagenesis approach for the generation and affinity maturation of Anti-CD20 nanobody.

Mohammad Mehdi HeidariElham Anbar ShiraziSeyedeh Fatemeh CheraghiRoghayeh ShahshahaniTina RahnamaMehri Khatami
Published in: Molecular biology reports (2024)
The approach we followed in this study makes it possible to create multiple anti-CD20 nanobodies with varying affinities without the need for extensive selection efforts. Additionally, our research has demonstrated that computational tools are highly reliable in designing functional nanobodies.
Keyphrases
  • crispr cas
  • quality improvement
  • mass spectrometry