CDR grafting and site-directed mutagenesis approach for the generation and affinity maturation of Anti-CD20 nanobody.
Mohammad Mehdi HeidariElham Anbar ShiraziSeyedeh Fatemeh CheraghiRoghayeh ShahshahaniTina RahnamaMehri KhatamiPublished in: Molecular biology reports (2024)
The approach we followed in this study makes it possible to create multiple anti-CD20 nanobodies with varying affinities without the need for extensive selection efforts. Additionally, our research has demonstrated that computational tools are highly reliable in designing functional nanobodies.