Early detection of Ebola virus proteins in peripheral blood mononuclear cells from infected mice.
Michael D WardTara KennyErnie BruggemanChristopher D KaneCourtney L MorrellMolly M KaneSandra BixlerSarah L GradyRachel S QuizonMekbib AstatkeLisa H CazaresPublished in: Clinical proteomics (2020)
While mass spectrometry is not currently amenable to field diagnostics, these results suggest that viral protein enrichment in PBMCs in tandem with highly sensitive immunoassays platforms, could lead to the development of a rapid, high-throughput diagnostic platform for pre-symptomatic detection of EBOV infection.
Keyphrases
- high throughput
- loop mediated isothermal amplification
- mass spectrometry
- label free
- liquid chromatography
- single cell
- sars cov
- high fat diet induced
- sensitive detection
- gas chromatography
- protein protein
- high performance liquid chromatography
- real time pcr
- capillary electrophoresis
- high resolution
- binding protein
- amino acid
- molecularly imprinted
- small molecule
- ms ms
- wild type
- insulin resistance
- metabolic syndrome
- solid phase extraction