Secretome derived from breast tumor cell lines alters the morphology of human umbilical vein endothelial cells.
Erika Olivia GómezYolanda Irasema ChirinoNorma Laura Delgado-BuenrostroAlejandro López-SaavedraNoemí Meraz-CruzRebeca López-MarurePublished in: Molecular membrane biology (2016)
Metastases, responsible for most of the solid tumor associated deaths, require angiogenesis and changes in endothelial cells. In this work, the effect of the secretomes of three breast tumor cell lines (MCF-7, MDA-MB-231 and ZR-75-30) on human umbilical vein endothelial cells (HUVEC) morphology was investigated. HUVEC treated with secretomes from breast cells were analyzed by confocal and time-lapse microscopy. Secretomes from ZR-75-30 and MDA-MB-231 cells modify the morphology and adhesion of HUVEC. These changes may provoke the loss of endothelial monolayer integrity. In consequence, tumor cells could have an increased access to circulation, which would then enhance metastasis.
Keyphrases
- endothelial cells
- cell cycle arrest
- induced apoptosis
- high glucose
- breast cancer cells
- vascular endothelial growth factor
- cell death
- optical coherence tomography
- pet imaging
- pi k akt
- oxidative stress
- signaling pathway
- mass spectrometry
- escherichia coli
- newly diagnosed
- cystic fibrosis
- cell proliferation
- high speed
- atomic force microscopy
- candida albicans
- label free
- cell adhesion