In Situ Silver-Based Electrochemical Oncolytic Bioreactor.
Yong HuangLiping ZhongXiaotong LiPan WuJian HeChao TangZhiping TangJing SuZhenbo FengBing WangYun MaHongmei PengZhihao BaiYi ZhongYing LiangWenxi LuRuiyu LuoJinghua LiHaiping LiZhiming DengXianli LanZiqun LiuKun ZhangYongxiang ZhaoPublished in: Advanced materials (Deerfield Beach, Fla.) (2022)
In this study, it is shown for the first time that a reduced graphene oxide (rGO) carrier has a 20-fold higher catalysis rate than graphene oxide in Ag + reduction. Based on this, a tumor microenvironment-enabled in situ silver-based electrochemical oncolytic bioreactor (SEOB) which switched Ag + prodrugs into in situ therapeutic silver nanoparticles with and above 95% transition rate is constructed to inhibit the growths of various tumors. In this SEOB-enabled intratumoral nanosynthetic medicine, intratumoral H 2 O 2 and rGO act as the reductant and the catalyst, respectively. Chelation of aptamers to the SEOB-unlocked prodrugs increases the production of silver nanoparticles in tumor cells, especially in the presence of Vitamin C, which is broken down in tumor cells to supply massive amounts of H 2 O 2 . Consequently, apoptosis and pyroptosis are induced to cooperatively contribute to the considerably-elevated anti-tumor effects on subcutaneous HepG2 and A549 tumors and orthotopic implanted HepG2 tumors in livers of nude mice. The specific aptamer targeting and intratumoral silver nanoparticle production guarantee excellent biosafety since it fails to elicit tissue damages in monkeys, which greatly increases the clinical translation potential of the SEOB system.
Keyphrases
- silver nanoparticles
- reduced graphene oxide
- gold nanoparticles
- wastewater treatment
- visible light
- oxidative stress
- diabetic rats
- ionic liquid
- cell death
- label free
- endoplasmic reticulum stress
- highly efficient
- cell cycle arrest
- drug delivery
- signaling pathway
- cancer therapy
- high resolution
- sensitive detection
- liquid chromatography
- electron transfer
- simultaneous determination
- skeletal muscle
- stress induced