A lanthanide tag for a complementary set of pseudocontact shifts.

Lydia ToppingAdarshi P WelegedaraMartyna JuddElwy H Abdelkader Nicholas Cox Gottfried Otting Stephen J Butler

Published in: Chemical communications (Cambridge, England) (2024)

Pseudocontact shifts (PCS) generated by paramagnetic lanthanide ions deliver powerful restraints for protein structure analysis by NMR spectroscopy. We present a new lanthanide tag that generates different PCSs than that of a related tag, which differs in structure by a single oxygen atom. It is highly reactive towards cysteine and performs well in turn-on luminescence and in EPR spectroscopy.

Keyphrases

single molecule
energy transfer
living cells
quantum dots
metal organic framework
fluorescent probe
sensitive detection
binding protein
drug induced
data analysis