Acetylcholine Structure-Based Small Activatable Fluorogenic Probe for Specific Detection of Acetylcholinesterase.
Jagpreet S SidhuKalpana RajendranAbraham B MathewTabish IqbalDeepak Kumar SainiDebasis DasPublished in: Analytical chemistry (2023)
Early detection of Alzheimer's disease (AD) is important for taking proper measures against AD pathogenesis. Acetylcholinesterase (AChE) is widely reported to be associated with the pathogenicity of AD. Here, employing the "acetylcholine-mimic" approach, we designed and synthesized a new class of naphthalimide (Naph)-based fluorogenic probes for specific detection of AChE and avoiding interference of butyrylcholinesterase (BuChE), the pseudocholinesterase. We investigated the action of the probes on Electrophorus electricus AChE, and the native human brain AChE that we expressed in Escherichia coli and purified in the active form for the first time. The probe Naph-3 exhibited a substantial fluorescence enhancement with AChE and majorly avoided BuChE. Naph-3 successfully crossed the cell membrane of the Neuro-2a cells and fluoresced upon reaction with endogenous AChE. We further established that the probe could be effectively used for screening AChE inhibitors. Our study provides a new avenue for the specific detection of AChE, which can be extended to the diagnosis of AChE-related complications.
Keyphrases
- living cells
- escherichia coli
- quantum dots
- fluorescent probe
- real time pcr
- small molecule
- induced apoptosis
- loop mediated isothermal amplification
- label free
- oxidative stress
- multidrug resistant
- staphylococcus aureus
- mass spectrometry
- fluorescence imaging
- biofilm formation
- energy transfer
- cognitive decline
- endoplasmic reticulum stress