Detection of neutralizing antibodies against multiple SARS-CoV-2 strains in dried blood spots using cell-free PCR.
Kenneth DanhDonna Grace KarpMalvika SinghalAkshaya TankasalaDavid GebhartFelipe de Jesus CortezDevangkumar TandelPeter V RobinsonDavid SeftelMars StoneGraham SimmonsAnil BagriMartin A SchreiberAndreas S BuserAndreas HolbroManuel BattegayMary Kate MorrisCarl HansonJohn R MillsDane GrangerElitza S TheelJames R StubbsLaurence M CorashCheng-Ting TsaiPublished in: Nature communications (2022)
An easily implementable serological assay to accurately detect severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) neutralizing antibodies is urgently needed to better track herd immunity, vaccine efficacy and vaccination rates. Herein, we report the Split-Oligonucleotide Neighboring Inhibition Assay (SONIA) which uses real-time qPCR to measure the ability of neutralizing antibodies to block binding between DNA-barcoded viral spike protein subunit 1 and the human angiotensin-converting enzyme 2 receptor protein. The SONIA neutralizing antibody assay using finger-prick dried blood spots displays 91-97% sensitivity and 100% specificity in comparison to the live-virus neutralization assays using matched serum specimens for multiple SARS-CoV-2 variants-of-concern. The multiplex version of this neutralizing antibody assay, using easily collectable finger-prick dried blood spots, can be a valuable tool to help reveal the impact of age, pre-existing health conditions, waning immunity, different vaccination schemes and the emergence of new variants-of-concern.
Keyphrases
- sars cov
- respiratory syndrome coronavirus
- high throughput
- cell free
- dengue virus
- angiotensin converting enzyme
- real time pcr
- binding protein
- circulating tumor
- healthcare
- copy number
- zika virus
- angiotensin ii
- public health
- escherichia coli
- single cell
- mental health
- coronavirus disease
- genome wide
- health information
- transcription factor
- aedes aegypti
- single molecule
- circulating tumor cells
- climate change