Unlocking the Potential of Carbon Quantum Dots for Cell Imaging, Intracellular Localization, and Gene Expression Control in Arabidopsis thaliana (L.) Heynh.
Zhimin LinMuhammad Moaaz AliXiaoyan YiLijuan ZhangShaojuan WangFaxing ChenPublished in: International journal of molecular sciences (2023)
Utilizing carbon quantum dots (CQDs) as biomaterials for delivering small substances has gained significant attention in recent research. However, the interactions and mechanisms of action of CQDs on plants have received relatively little focus. Herein, we investigated the transportation of CQDs into various organs of Arabidopsis thaliana (L.) Heynh. via the vessel system, leading to the epigenetic inheritance of Argonaute family genes. Our findings reveal that CQDs may interact with microRNAs (miRNAs), leading to the repression of post-transcriptional regulation of target genes in the cytoplasm. Transcriptome and quantitative PCR analyses demonstrated consistent gene expression levels in offspring. Moreover, microscopic observations illustrated rapid CQD localization on cell membranes and nuclei, with increased nuclear entry at higher concentrations. Notably, our study identified an alternative regulatory microRNA, microRNA172D, for the Argonaute family genes through methylation analysis, shedding light on the connection between CQDs and microRNAs.
Keyphrases
- arabidopsis thaliana
- gene expression
- genome wide
- dna methylation
- quantum dots
- single cell
- high resolution
- bioinformatics analysis
- cell therapy
- rna seq
- genome wide identification
- sensitive detection
- copy number
- stem cells
- transcription factor
- working memory
- high fat diet
- drinking water
- adipose tissue
- photodynamic therapy
- insulin resistance