Effects of Bioreactor-Oxygenation During Extracorporeal Granulocytes Treatment in Septic Patients.
Martin SauerGeorg RichterJens AltrichterThomas WildFanny DoßThomas MenckeJohannes EhlerSandra DoßStephanie KochAnton SchubertGabriele Nöldge-SchomburgSteffen R MitznerPublished in: Therapeutic apheresis and dialysis : official peer-reviewed journal of the International Society for Apheresis, the Japanese Society for Apheresis, the Japanese Society for Dialysis Therapy (2018)
A granulocyte bioreactor for the extracorporeal treatment was developed to enhance the immune cell function in patients with severe sepsis. The influence of oxygenation on the used cells was tested in a prospective clinical study. Ten patients with severe sepsis were treated twice with the granulocyte bioreactor. The used cells were screened for functionality; values of blood gases, glucose and lactate were obtained from the recirculating bioreactor circuit. Five patients were treated with an oxygenator setup (Oxy group), five without oxygenator (Non-Oxy group). The overall in-hospital mortality was 50%. Significantly lower values of oxygen saturation, partial oxygen pressure, lactate, oxyburst and phagocytosis were seen in the Non-Oxy group compared with the Oxy group in the bioreactor circuit. Further studies with this approach are encouraged and should focus on the influence of oxygenation on production of reactive oxygen species and cytokines of used cells.
Keyphrases
- induced apoptosis
- wastewater treatment
- newly diagnosed
- end stage renal disease
- cell cycle arrest
- acute kidney injury
- chronic kidney disease
- reactive oxygen species
- intensive care unit
- prognostic factors
- early onset
- metabolic syndrome
- blood pressure
- cell death
- clinical trial
- type diabetes
- endoplasmic reticulum stress
- adipose tissue
- blood flow
- patient reported outcomes
- drug induced
- replacement therapy