Immunogenicity and protective efficacy of recombinant adenovirus expressing a novel genotype G2b PEDV spike protein in protecting newborn piglets against PEDV.
Xin SongQun ZhouJiaqi ZhangTaoyun ChenGunan DengHua YueCheng TangXuejing WuJifeng YuBin ZhangPublished in: Microbiology spectrum (2023)
Porcine epidemic diarrhea (PED) is a highly infectious and economically significant gastrointestinal disorder that affects pigs of all ages. Preventing and controlling PED is achieved by immunizing sows with vaccines, enabling passive piglet immunization via colostrum. The prevalence of G2b porcine epidemic diarrhea virus (PEDV) continues in China despite the use of commercial vaccines, raising questions regarding current vaccine efficacy and the need for novel vaccine development. Adenovirus serotype 5 (Ad5) has several advantages, including high transduction efficiency, a wide range of host cells, and the ability to infect cells at various stages. In this study, we expressed the immunogenic proteins of spike (S) using an Ad5 vector and generated a PED vaccine candidate by inducing significant humoral immunity. The rAd5-PEDV-S prevented PED-induced weight loss, diarrhea, and intestinal damage in piglets. This novel vaccine candidate strain possesses the potential for use in the pig breeding industry.
Keyphrases
- induced apoptosis
- weight loss
- irritable bowel syndrome
- oxidative stress
- immune response
- clostridium difficile
- type diabetes
- endoplasmic reticulum stress
- bariatric surgery
- risk factors
- dna repair
- diabetic rats
- gene therapy
- dengue virus
- high glucose
- adipose tissue
- body mass index
- signaling pathway
- endothelial cells
- risk assessment
- zika virus
- stress induced
- klebsiella pneumoniae
- drug induced
- amino acid