ICM conversion to epiblast by FGF/ERK inhibition is limited in time and requires transcription and protein degradation.
Sylvain BessonnardSabrina CoqueranSandrine Vandormael-PourninAlexandre C DufourJérôme ArtusMichel Cohen-TannoudjiPublished in: Scientific reports (2017)
Inner cell Mass (ICM) specification into epiblast (Epi) and primitive endoderm (PrE) is an asynchronous and progressive process taking place between E3.0 to E3.75 under the control of the Fibroblast Growth Factor (FGF)/Extracellular signal-Regulated Kinase (ERK) signaling pathway. Here, we have analyzed in details the kinetics of specification and found that ICM cell responsiveness to the up and down regulation of FGF signaling activity are temporally distinct. We also showed that PrE progenitors are generated later than Epi progenitors. We further demonstrated that, during this late phase of specification, a 4 hours period of FGF/ERK inhibition prior E3.75 is sufficient to convert ICM cells into Epi. Finally, we showed that ICM conversion into Epi in response to inhibition during this short time window requires both transcription and proteasome degradation. Collectively, our data give new insights into the timing and mechanisms involved in the process of ICM specification.
Keyphrases
- signaling pathway
- pi k akt
- induced apoptosis
- cell fate
- cell cycle arrest
- cell proliferation
- single cell
- transcription factor
- cell therapy
- epithelial mesenchymal transition
- multiple sclerosis
- stem cells
- tyrosine kinase
- machine learning
- endoplasmic reticulum stress
- cell death
- mesenchymal stem cells
- oxidative stress
- binding protein
- bone marrow
- protein protein
- embryonic stem cells