UV Treatment Improves the Biocompatibility and Antibacterial Properties of Crystallized Nanostructured Titanium Surface.
Mai HatokoSatoshi KomasaHonghao ZhangTohru SekinoJoji OkazakiPublished in: International journal of molecular sciences (2019)
This study describes the production of a new material composed of pure titanium (Ti) metal with a crystallized nanostructure and investigated whether heat treatment and ultraviolet (UV) irradiation improved its biocompatibility and antibacterial properties. We compared the performance of UV-irradiated and non-irradiated Ti nanosheets (TNS) formed by dark alkaline treatment and heating at 600 °C with that of untreated pure Ti nanostructure (positive control). In vitro and in vivo experiments to assess biocompatibility and effects on cell behavior were performed using human umbilical vein endothelial cells and rat bone marrow cells. The material surface was characterized by X-ray photoelectron spectroscopy (XPS). The antibacterial properties of the irradiated material were evaluated using Staphylococcus aureus, a common pathogenic bacterium. The UV-irradiated TNS exhibited high angiogenic capacity and promoted cell adherence and differentiation relative to the control. Further, surface analysis via XPS revealed a lower C peak for the UV-treated material, indicating a reduced amount of dirt on the material surface. Moreover, UV irradiation decreased the viability of S. aureus on the material surface by stimulating reactive oxygen species production. The biocompatibility and antibacterial properties of the TNS were improved by UV irradiation. Thus, TNS may serve as a useful material for fabrication of dental implants.
Keyphrases
- staphylococcus aureus
- bone marrow
- endothelial cells
- single cell
- high resolution
- cell therapy
- induced apoptosis
- stem cells
- oxidative stress
- mesenchymal stem cells
- silver nanoparticles
- magnetic resonance imaging
- single molecule
- insulin resistance
- anti inflammatory
- metabolic syndrome
- endoplasmic reticulum stress
- quantum dots
- cell death
- combination therapy
- candida albicans
- radiation induced
- vascular endothelial growth factor
- reduced graphene oxide
- pseudomonas aeruginosa
- essential oil
- wound healing
- weight loss
- cell cycle arrest
- glycemic control