Comparison of quantitative real-time PCR and direct immunofluorescence for the detection of Pneumocystis jirovecii.
Bhavani MoodleyStefano TempiaJohn Andrew FreanPublished in: PloS one (2017)
The results of the ROC curve analysis indicate an excellent predictive value of the qPCR using the proposed cut-off. However, the IFA test is an imperfect gold standard and so this cut-off should not be used in isolation; clinical data should also contribute to the interpretation of the qPCR result.