Optimization by infusion of multiple reaction monitoring transitions for sensitive quantification of peptides by liquid chromatography/mass spectrometry.
Bandar AlghanemFrédéric NikitinThomas StrickerEva DuchoslavJeremy LubanCaterina Strambio-De-CastillaMarkus MullerFrédérique LisacekEmmanuel VaresioGérard HopfgartnerPublished in: Rapid communications in mass spectrometry : RCM (2018)
Isotopically labelled peptides can be used to optimize MRM transitions more efficiently in FIA than by searching databases. The MRMOptimizer software is MS independent and enables the post-acquisition selection of MRM parameters. Coefficients of variation for optimal CE values are lower than those obtained with the SRMAtlas approach (B2) and one additional peptide was detected. Copyright © 2017 John Wiley & Sons, Ltd.
Keyphrases
- mass spectrometry
- liquid chromatography
- high resolution mass spectrometry
- tandem mass spectrometry
- gas chromatography
- high performance liquid chromatography
- capillary electrophoresis
- amino acid
- high resolution
- simultaneous determination
- low dose
- solid phase extraction
- ms ms
- big data
- data analysis
- deep learning
- energy transfer