A responsive fluorescent probe for detecting and imaging pyruvate kinase M2 in live cells.

Ya ZhangHuishan LiHailing MaiDong LuoXinpei JiZhengting LiuShiyong Peng Xue-Tao XuYinghui ZhangRongfeng Lan Hongguang Li

Published in: Chemical communications (Cambridge, England) (2022)

In this study, we designed and tested fluorescent probe zy-2 for specific and responsive imaging of pyruvate kinase M2 (PKM2), which can be excited by 419 nm light. A 17-fold enhancement in the responsive emission upon zy-2's binding to PKM2 was observed, imaging of which was successfully recorded in a time- and concentration-dependent manner in PKM2-positive cells. Thus, we obtained a responsive fluorescent probe for the specific and sensitive detection of PKM2, which is innovative in design and applicable to the detection of cancer cells.

Keyphrases

fluorescent probe
living cells
sensitive detection
high resolution
induced apoptosis
cancer therapy
cell cycle arrest
loop mediated isothermal amplification
quantum dots
photodynamic therapy
signaling pathway
protein kinase
oxidative stress
fluorescence imaging
mass spectrometry
energy transfer