Angiotensin II Treatment Induces Reorganization and Changes in the Lateral Dynamics of Angiotensin II Type 1 Receptor in the Plasma Membrane Elucidated by Photoactivated Localization Microscopy Combined with Image Spatial Correlation Analysis.
Camilla AndersenYenisleidy de Las Mercedes Zulueta DíazJakob L KureMathias Hessellund EriksenAdam Leslie LovattChristoffer LagerholmSebastian MoralesSimon SehayekThomas M D SheardPaul W WisemanEva C ArnspangPublished in: Analytical chemistry (2022)
The mechanisms by which angiotensin II type 1 receptor is distributed and the diffusional pattern in the plasma membrane (PM) remain unclear, despite their crucial role in cardiovascular homeostasis. In this work, we obtained quantitative information of angiotensin II type 1 receptor (AT1R) lateral dynamics as well as changes in the diffusion properties after stimulation with ligands in living cells using photoactivated localization microscopy (PALM) combined with image spatial-temporal correlation analysis. To study the organization of the receptor at the nanoscale, expansion microscopy (ExM) combined with PALM was performed. This study revealed that AT1R lateral diffusion increased after binding to angiotensin II (Ang II) and the receptor diffusion was transiently confined in the PM. In addition, ExM revealed that AT1R formed nanoclusters at the PM and the cluster size significantly decreased after Ang II treatment. Taking these results together suggest that Ang II binding and activation cause reorganization and changes in the dynamics of AT1R at the PM.
Keyphrases
- angiotensin ii
- angiotensin converting enzyme
- vascular smooth muscle cells
- particulate matter
- single molecule
- air pollution
- living cells
- high resolution
- polycyclic aromatic hydrocarbons
- fluorescent probe
- heavy metals
- minimally invasive
- high throughput
- binding protein
- high speed
- single cell
- label free
- water soluble
- mass spectrometry
- social media
- sensitive detection
- health information
- transcription factor
- data analysis