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IMPLANT: a new technique for transgene copy number estimation in plants using a single end-point PCR reaction.

Jonas De SaegerJihae ParkKai ThorisCharlotte De BruynHoo Sun ChungDirk InzéStephen Depuydt
Published in: Plant methods (2022)
We show that this technique is not only reliable, but is also faster, easier, and cheaper as compared with other techniques. Accurate results are obtained in both Arabidopsis and rice, but this technique can be easily extended to other organisms and as such can be widely adopted in the field of biotechnology.
Keyphrases
  • copy number
  • mitochondrial dna
  • transcription factor
  • genome wide
  • gram negative
  • soft tissue
  • high resolution
  • dna methylation
  • real time pcr
  • cell wall
  • plant growth
  • multidrug resistant
  • electron transfer
  • gene expression