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New insights into the structure and function of chemokine receptor:chemokine complexes from an experimental perspective.

Martin Gustavsson
Published in: Journal of leukocyte biology (2020)
Chemokines are small soluble proteins that drive cell migration through the formation of concentration gradients. Chemokine binding to G protein-coupled chemokine receptors in the cell membrane activates intracellular signaling pathways and is a fundamental process involved in numerous physiological and pathophysiological functions. In the past few years, significant experimental developments have made it possible to characterize complexes between chemokine receptors and chemokines at a molecular level. Here, I review these developments from an experimental perspective, focusing on how the ability to express, purify, and stabilize receptor:chemokine complexes have made studies by X-ray crystallography, nuclear magnetic resonance, and other methods possible. I give examples of how these studies have advanced our understanding of the architecture of receptor:chemokine complexes as well as the mechanisms involved in complex formation. Finally, I discuss some of the many remaining questions and challenges that will require studies of more receptors and chemokines as well as further development of experimental methods.
Keyphrases
  • magnetic resonance
  • cell migration
  • signaling pathway
  • case control
  • magnetic resonance imaging
  • high resolution
  • binding protein
  • reactive oxygen species
  • contrast enhanced
  • pi k akt
  • dual energy