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Modern Solutions for Ancient Pathogens: Direct Pathogen Sequencing for Diagnosis of Lepromatous Leprosy and Cerebral Coenurosis.

Bernadette C YoungStephen J BushSam LipworthSophie GeorgeKate E DingleNick SandersonAlice BrankinTimothy WalkerSrilakshmi SharmaJames LeongPuneet PlahaMonika HoferPeter ChiodiniB GottsteinLavinia FurrerDerrick CrookAndrew Brent
Published in: Open forum infectious diseases (2022)
Microbes unculturable in vitro remain diagnostically challenging, dependent historically on clinical findings, histology, or targeted molecular detection. We applied whole-genome sequencing directly from tissue to diagnose infections with mycobacteria (leprosy) and parasites (coenurosis). Direct pathogen DNA sequencing provides flexible solutions to diagnosis of difficult pathogens in diverse contexts.
Keyphrases
  • single cell
  • gram negative
  • candida albicans
  • antimicrobial resistance
  • circulating tumor
  • cancer therapy
  • cell free
  • multidrug resistant
  • plasmodium falciparum
  • brain injury
  • cerebral blood flow