Verbascoside Protects Pancreatic β-Cells against ER-Stress.
Alessandra GalliPaola MarcianiAlgerta MarkuSilvia GhislanzoniFederico BertuzziRaffaella RossiAlessia Di GiancamilloMichela CastagnaCarla PeregoPublished in: Biomedicines (2020)
Substantial epidemiological evidence indicates that a diet rich in polyphenols protects against developing type 2 diabetes. The phenylethanoid glycoside verbascoside/acteoside, a widespread polyphenolic plant compound, has several biological properties including strong antioxidant, anti-inflammatory and neuroprotective activities. The aim of this research was to test the possible effects of verbascoside on pancreatic β-cells, a target never tested before. Mouse and human β-cells were incubated with verbascoside (0.8-16 µM) for up to five days and a combination of biochemical and imaging techniques were used to assess the β-cell survival and function under normal or endoplasmic reticulum (ER)-stress inducing conditions. We found a dose-dependent protective effect of verbascoside against oxidative stress in clonal and human β-cells. Mechanistic studies revealed that the polyphenol protects β-cells against ER-stress mediated dysfunctions, modulating the activation of the protein kinase RNA-like endoplasmic reticulum kinase (PERK) branch of the unfolded protein response and promoting mitochondrial dynamics. As a result, increased viability, mitochondrial function and insulin content were detected in these cells. These studies provide the evidence that verbascoside boosts the ability of β-cells to cope with ER-stress, an important contributor of β-cell dysfunction and failure in diabetic conditions and support the therapeutic potential of verbascoside in diabetes.
Keyphrases
- induced apoptosis
- type diabetes
- oxidative stress
- endoplasmic reticulum
- cell cycle arrest
- endoplasmic reticulum stress
- cardiovascular disease
- signaling pathway
- anti inflammatory
- cell death
- dna damage
- glycemic control
- physical activity
- cell proliferation
- mesenchymal stem cells
- weight loss
- brain injury
- bone marrow
- small molecule
- wound healing