Surface-Bound Gradient Deposition of Protein Nanoparticles for Cell Motility Studies.
Witold I TatkiewiczJoaquin Seras-FranzosoElena Garcia-FruitósEsther VazquezA R KyvikJudith GuaschAntonio VillaverdeJaume VecianaImma RateraPublished in: ACS applied materials & interfaces (2018)
A versatile evaporation-assisted methodology based on the coffee-drop effect is described to deposit nanoparticles on surfaces, obtaining for the first time patterned gradients of protein nanoparticles (pNPs) by using a simple custom-made device. Fully controllable patterns with specific periodicities consisting of stripes with different widths and distinct nanoparticle concentration as well as gradients can be produced over large areas (∼10 cm2) in a fast (up to 10 mm2/min), reproducible, and cost-effective manner using an operational protocol optimized by an evolutionary algorithm. The developed method opens the possibility to decorate surfaces "a-la-carte" with pNPs enabling different categories of high-throughput studies on cell motility.
Keyphrases
- biofilm formation
- single cell
- high throughput
- cell therapy
- machine learning
- protein protein
- pseudomonas aeruginosa
- amino acid
- deep learning
- gene expression
- staphylococcus aureus
- genome wide
- small molecule
- candida albicans
- stem cells
- binding protein
- bone marrow
- walled carbon nanotubes
- mesenchymal stem cells
- cystic fibrosis
- neural network