Comprehensive off-target analysis of dCas9-SAM-mediated HIV reactivation via long noncoding RNA and mRNA profiling.

Yonggang ZhangGustavo ArangoFang LiXiao XiaoRaj PutatundaJun YuXiao-Feng YangHong WangLayne T WatsonLiqing ZhangWenhui Hu

Published in: BMC medical genomics (2018)

This is an extremely comprehensive study demonstrating the rare off-target effects of the HIV-specific dCas9-SAM system in human cells. This finding is encouraging for the safe application of dCas9-SAM technology to induce target-specific reactivation of latent HIV for an effective "shock-and-kill" strategy.

Keyphrases

antiretroviral therapy
hiv positive
hiv testing
hiv infected
long noncoding rna
human immunodeficiency virus
hepatitis c virus
hiv aids
men who have sex with men
south africa
single cell