Estimation of the bacteriocin ColE7 conjugation-based "kill" - "anti-kill" antimicrobial system by real-time PCR, fluorescence staining and bioluminescence assays.
I L MaslennikovaM V KuznetsovaN ToplakI V NekrasovaD Žgur BertokMateja Erdani KreftPublished in: Letters in applied microbiology (2018)
Bacterial antimicrobial resistance is worldwide rising and causing deaths of thousands of patients infected with multi-drug resistant bacterial strains. In addition, there is a lack of efficient alternative antimicrobial agents. The significance of our research is the use of a number of methods (real-time PCR, flow cytometry and bioluminescence-based technique) to assess the antibacterial activity of the bacteriocin, colicin ColE7, bacterial conjugation-based "kill" - "anti-kill" antimicrobial system. Bioluminescent techniques proved to be rapid and suitable for estimation of antibacterial activity of ColE7 bacterial conjugation-based antimicrobial system and possibly other related systems.
Keyphrases
- real time pcr
- drug resistant
- flow cytometry
- staphylococcus aureus
- antimicrobial resistance
- end stage renal disease
- multidrug resistant
- acinetobacter baumannii
- newly diagnosed
- ejection fraction
- chronic kidney disease
- peritoneal dialysis
- prognostic factors
- high throughput
- cystic fibrosis
- silver nanoparticles
- patient reported outcomes
- quantum dots
- pseudomonas aeruginosa
- drug induced
- loop mediated isothermal amplification