Login / Signup

Intra-host growth kinetics of dengue virus in the mosquito Aedes aegypti.

Mario NoveloMatthew D HallDamie PakPaul R YoungEdward C HolmesElizabeth A McGraw
Published in: PLoS pathogens (2019)
Dengue virus (DENV) transmission by mosquitoes is a time-dependent process that begins with the consumption of an infectious blood-meal. DENV infection then proceeds stepwise through the mosquito from the midgut to the carcass, and ultimately to the salivary glands, where it is secreted into saliva and then transmitted anew on a subsequent bite. We examined viral kinetics in tissues of the Aedes aegypti mosquito over a finely graded time course, and as per previous studies, found that initial viral dose and serotype strain diversity control infectivity. We also found that a threshold level of virus is required to establish body-wide infections and that replication kinetics in the early and intermediate tissues do not predict those of the salivary glands. Our findings have implications for mosquito GMO design, modeling the contribution of transmission to vector competence and the role of mosquito kinetics in the overall DENV epidemiological landscape.
Keyphrases
  • dengue virus
  • aedes aegypti
  • zika virus
  • gene expression
  • sars cov
  • single cell
  • case control