Gold Nanoflares with Computing Function as Smart Diagnostic Automata for Multi-miRNA Patterns in Living Cells.
Lan LiuNa LiZhi-Mei HuangLi-Juan TangZhan-Ming YingJian-Hui JiangPublished in: Analytical chemistry (2020)
Investigating the multimolecule patterns in living cells is of vital importance for clinical and biomedical studies. Herein, we reported for the first time the engineering of gold nanoflares as smart automata to implement computing-based diagnosis in living mammalian cells. Defining the logic combinations of miR122 and miR21 as the detection patterns, the corresponding OR and AND diagnostic automata were designed. The results showed that they could recognize the correct patterns rapidly and sensitively. The automata could enter cells via self-delivery and have good biocompatibility. They enabled accurate diagnosis on miRNA signatures in different cell lines and differentiation of fluctuations in the same cell line at single cell resolution. Moreover, the automata afforded an innovative diagnostic mode. It simplified the complicated process of detecting, data-collecting, computing, and evaluating. The direct diagnosing result ("1" or "0") was exported according to the embedded computation code. It highlighted the new possibility of using smart automata for intelligent diagnostics and cancer therapy at single cell resolution.
Keyphrases
- living cells
- fluorescent probe
- single molecule
- single cell
- cancer therapy
- cell proliferation
- long non coding rna
- rna seq
- induced apoptosis
- drug delivery
- long noncoding rna
- high throughput
- oxidative stress
- high resolution
- big data
- gene expression
- cell cycle arrest
- cell death
- silver nanoparticles
- loop mediated isothermal amplification
- real time pcr
- mass spectrometry