Multiplexed live-cell imaging for drug responses in patient-derived organoid models of cancer.

Kaitriana E CollingEmily L SymonsLorenzo BuroniHiruni K SumanisiriJessica Andrew-UdohEmily WittHaley A LoshAbigail M MorrisonKimberly K LeslieChristopher J DunnillJohann S De BonoKristina W W Thiel

Published in: bioRxiv : the preprint server for biology (2023)

Patient-derived tumor organoids are a sophisticated model system for basic and translational research. This methods article details the use of multiplexed fluorescent live-cell imaging for simultaneous kinetic assessment of different organoid phenotypes.

Keyphrases

high resolution
single cell
squamous cell carcinoma
living cells
fluorescence imaging
childhood cancer