Amphotericin B-Silver Hybrid Nanoparticles Help to Unveil the Mechanism of Biological Activity of the Antibiotic: Disintegration of Cell Membranes.
Sebastian JanikEwa GrelaSylwia StączekAgnieszka Zdybicka-BarabasRafal LuchowskiWieslaw I GruszeckiWojciech GrudzińskiPublished in: Molecules (Basel, Switzerland) (2023)
Amphotericin B is a popular antifungal antibiotic, and despite decades of pharmacological application, the exact mode of its biological activity is still a matter of debate. Amphotericin B-silver hybrid nanoparticles (AmB-Ag) have been reported to be an extremely effective form of this antibiotic to combat fungi. Here, we analyze the interaction of AmB-Ag with C. albicans cells with the application of molecular spectroscopy and imaging techniques, including Raman scattering and Fluorescence Lifetime Imaging Microscopy. The results lead to the conclusion that among the main molecular mechanisms responsible for the antifungal activity of AmB is the disintegration of the cell membrane, which occurs on a timescale of minutes.
Keyphrases
- high resolution
- single molecule
- gold nanoparticles
- candida albicans
- induced apoptosis
- quantum dots
- single cell
- silver nanoparticles
- cell cycle arrest
- mass spectrometry
- high speed
- stem cells
- endoplasmic reticulum stress
- optical coherence tomography
- oxidative stress
- photodynamic therapy
- mesenchymal stem cells
- signaling pathway
- label free
- molecular dynamics