Development of a multiplex reverse transcription-quantitative PCR (qPCR) method for detecting common causative agents of swine viral diarrhea in China.

Wenbo SongYixue FengJiali ZhangDanni KongJie FanMengfei ZhaoLin HuaJinmei XiangXibiao TangShaobo XiaoZhong PengBin Wu

Published in: Porcine health management (2024)

A TaqMan multiplex qPCR method targeting PEDV, TGEV, PDCoV, and RVA was developed in this study. This method demonstrated a good specificity and sensitivity on investigating these four common viruses responsible for viral diarrhea on Chinese pig farms, which represents a convenient method for the monitoring and differential diagnosis of swine viral diarrhea.

Keyphrases

sars cov
real time pcr
irritable bowel syndrome
clostridium difficile
high throughput
transcription factor
cancer therapy