TGF-β Ligand Interactions in the Response of Caenorhabditis elegans to Bacterial Pathogens.

The Transforming Growth Factor beta (TGF-β) family consists of numerous secreted peptide growth factors that play significant roles in cell function, tissue patterning, and organismal homeostasis including wound repair and immunity. Typically studied as homodimers, these ligands have the potential to diversify their functions through ligand interactions that are synergistic, cooperative, additive, and/or antagonistic. In the nematode Caenorhabditis elegans there are only five TGF-β ligands, providing an opportunity to dissect ligand interactions in a smaller number of combinations than in vertebrates. As in vertebrates, these ligands can be divided into bone morphogenetic protein (BMP) and TGF-β/Activin subfamilies that predominantly signal through discrete signaling pathways. The BMP subfamily ligand DBL-1 has been well studied for its role in the innate immune response in C. elegans . Here we show that all five TGF-β ligands play a role in the immune response. We also demonstrate that multiple TGF-β ligands act cooperatively as part of this response. We show that the two BMP-like ligands - DBL-1 and TIG-2 - function independently of each other in the immune response, while TIG-2/BMP and the TGF-β/Activin-like ligand TIG-3 function cooperatively. We also show that in the response to bacterial pathogen, canonical DBL-1/BMP receptor and Smad signal transducers function, while components of the DAF-7 TGF-β/Activin signaling pathway do not play a role in survival. These results demonstrate a novel potential for BMP and TGF-β/Activin subfamily ligands to interact, and may provide a mechanism for distinguishing the developmental and homeostatic functions of these ligands from an acute response such as the innate immune response to bacterial pathogens.