In Vitro Effect of Photodynamic Therapy with Indocyanine Green Followed by 660 nm Photobiomodulation Therapy on Fibroblast Viability.

This in␣vitro study sought to assess the effect of repetitive PBMT on the viability of fibroblasts following aPDT with indocyanine green (ICG). In this in␣vitro experimental study, human gingival fibroblasts (HGFs) were obtained and incubated in a culture medium. After reaching 10 000 cells cm-2 , the cells were divided into five groups of control, aPDT with ICG and 808 nm (energy density of 24 J cm-2 ), PBMT immediately after aPDT, PBMT with 660 nm (energy density of 7.2 J cm-2 ) immediately and 24 h after aPDT and PBMT immediately and 24 and 48 h after aPDT in 48-well plates. Cell viability was evaluated using the methyl thiazolyl tetrazolium (MTT) assay after 1, 4 and 7 days of incubation. Statistical analyses were performed using one-way ANOVA. Cell viability significantly decreased in group 2 (P < 0.002). We observed no significant increase in cell viability at any time point in group 3 (P > 0.05). Cell viability significantly increased in groups 4 and 5 after the first day of incubation (P < 0.000). Emission of 660 nm as PBMT for two and three times along with passage of time would increase the viability of HGFs following aPDT with ICG.