Reversible DNA micro-patterning using the fluorous effect.

Gabriella E FlynnJamie M WithersGerard MaciasJustin R SperlingSarah L HenryJonathan M CooperGlenn A BurleyAlasdair W Clark

Published in: Chemical communications (Cambridge, England) (2018)

We describe a new method for the immobilisation of DNA into defined patterns with sub-micron resolution, using the fluorous effect. The method is fully reversible via a simple solvent wash, allowing the patterning, regeneration and re-patterning of surfaces with no degradation in binding efficiency following multiple removal/attachment cycles of different DNA sequences.

Keyphrases

circulating tumor
single molecule
cell free
stem cells
cell fate
nucleic acid
staphylococcus aureus
escherichia coli
ionic liquid
pseudomonas aeruginosa
cystic fibrosis
binding protein
genetic diversity