Optimizing microbiome sequencing for small intestinal aspirates: validation of novel techniques through the REIMAGINE study.
Gabriela Guimaraes Sousa LeiteWalter MoralesStacy WeitsmanShreya CellyGonzalo ParodiRuchi MathurRashin SedighiGillian M BarlowAli RezaieMark PimentelPublished in: BMC microbiology (2019)
This study validates novel DTT-based methodology which optimizes microbial culture and 16S rRNA gene sequencing for the study of the small bowel microbiome. The microbial analyses indicate increased isolation of facultative and obligate anaerobes from the mucus layer using these novel techniques.