Evaluation of Xpert MTB/RIF Ultra Assay for Diagnosis of Childhood Tuberculosis: a Multicenter Accuracy Study.
Lin SunYu ZhuMin FangYan ShiXiaoshan PengQiong LiaoXingyun WangShuting QuanYacui WangLi DuanXiaomei ShiZhipeng ZhaoLanqin ChenYongsheng XuTongqiang ZhangXiaojian CuiChaomin WanAdong ShenPublished in: Journal of clinical microbiology (2020)
A multicenter study was performed to evaluate the value of testing gastric aspirate (GA) with Xpert MTB/RIF Ultra assay (Ultra) for childhood tuberculosis (TB) detection in China. In total, 129 children with active TB and 173 children without TB were enrolled. The sensitivity of Ultra in bacteriologically confirmed TB and probable TB cases was 87.5% (42/48) and 44.4% (36/81), respectively. The specificity of Ultra was high (99.4%, 172/173). When Ultra, culture, and acid-fast bacilli outcomes were integrated as a composite reference standard, the percentage of children with definite TB increased from 37.2% (48/129) to 67.4% (87/129). The sensitivity of Ultra is 80.0% (40/50) in children aged <4 years, which is significantly higher than that in older children (48.1%, 38/79) (P < 0.001). Ultra conducted using GA samples can provide faster results, allowing an early and accurate TB diagnosis, especially in younger children with difficulty producing sputum.
Keyphrases
- mycobacterium tuberculosis
- pulmonary tuberculosis
- high resolution
- young adults
- high throughput
- type diabetes
- metabolic syndrome
- physical activity
- cystic fibrosis
- emergency department
- skeletal muscle
- hepatitis c virus
- hiv aids
- mass spectrometry
- middle aged
- multidrug resistant
- hiv infected
- gram negative
- quantum dots
- structural basis