A new Trypanosoma cruzi genotyping method enables high resolution evolutionary analyses.
Christian Macagnan ProbstMyllena de Fátima Alheiros Dias MeloDaniela Parada PavoniMax Jean de Ornelas ToledoTainah Silva GaldinoAdeilton Alves BrandãoConstança BrittoMarco Aurelio KriegerPublished in: Memorias do Instituto Oswaldo Cruz (2021)
As the TS-LSS method is based on a single PCR reaction, comprising several copies of the target, it is probably useful for clinical samples, when the amount of DNA is a limiting factor. As large scale sequencing systems become more common, the TS-LSS method can be increasingly applied for T. cruzi genotyping.