Response Surface Methodology for Optimization of Process Parameters and Antioxidant Properties of Mulberry (Morus alba L.) Leaves by Extrusion.
Mina K KimDong-Geon NamWan-Taek JuJeong-Sook ChoeAe-Jin ChoiPublished in: Molecules (Basel, Switzerland) (2020)
Mulberry (Morus alba L.) leaves (MLs), originally used to feed silkworms, have recently been recognized as a food ingredient containing health-beneficial, bioactive compounds. In this study, the extrusion process was applied for the enhancement of the amount of extractable flavonoids from MLs. Extrusion conditions were optimized by water solubility index, total phenolic content, and total flavonoid content (TF) using response surface methodology, and antioxidative stress activities were evaluated in macrophage cells. According to the significance of regression coefficients of TF, the optimal extrusion parameters were set as barrel temperature of 114 °C, moisture feed content of 20%, and screw speed of 232 rpm. Under these conditions, the TF of extruded ML reached to 0.91% and improved by 63% compared with raw ML. Fifteen flavonoids were analyzed using ultra-high-performance liquid chromatograph coupled with photodiode array detection and quadrupole time-of-flight mass spectrometry (UPLC-PDA-QTOF/MS), and the extrusion resulted in increases in quercetin-3-gentiobioside, quercetin-3,7-di-O-glucoside, kaempferol-3,7-di-O-glucoside, rutin, isoquercitrin, and moragrol C. Besides, regarding antioxidative activity, extruded ML water extract inhibited the production of H2O2-induced reactive oxygen species and attenuated nuclear morphology alterations in macrophage cells. The findings of this study should be useful in food processing design to improve the extractable functional compounds in MLs.
Keyphrases
- induced apoptosis
- anti inflammatory
- mass spectrometry
- oxidative stress
- reactive oxygen species
- cell cycle arrest
- adipose tissue
- healthcare
- ms ms
- high resolution
- biofilm formation
- endoplasmic reticulum stress
- high throughput
- mental health
- signaling pathway
- high performance liquid chromatography
- liquid chromatography
- endothelial cells
- ionic liquid
- health information
- high glucose
- candida albicans
- label free
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- sensitive detection
- gas chromatography
- drug induced
- real time pcr
- finite element analysis