Injectable and Degradable PEG Hydrogel with Antibacterial Performance for Promoting Wound Healing.
Siju LiuTao JiangRenqi GuoChuang LiCuifen LuGuichun YangJunqi NieFeiyi WangXiaofan YangZhenbing ChenPublished in: ACS applied bio materials (2021)
Injectable and degradable PEG hydrogel was prepared via Michael-type addition between cross-linking monomer 4-arm-PEG-MAL and two cross-linkers of hydrolysis degradable PEG-diester-dithiol and non-degradable PEG-dithiol, and it had a porous structure with the uniform pore size. The biocompatibility assays in vitro indicated that PEG hydrogel had excellent biocompatibility and can be degraded naturally without leading to any negative impact on cells. The results of antibacterial experiments showed that PEG hydrogel can inhibit the growth of bacteria. Furthermore, the Cell Counting Kit-8 (CCK-8) assay, LIVE/DEAD cell staining, and scratch healing experiments proved that PEG hydrogel can promote cell proliferation and migration, which had been further confirmed in in vivo experiments on the rat wound models. All experimental results demonstrated that PEG hydrogel is an injectable antibacterial dressing, which can promote the process of wound healing and has great potential in the field of wound healing.
Keyphrases
- wound healing
- drug delivery
- tissue engineering
- hyaluronic acid
- single cell
- cell therapy
- high throughput
- oxidative stress
- induced apoptosis
- mesenchymal stem cells
- cell death
- risk assessment
- climate change
- silver nanoparticles
- cell cycle arrest
- mass spectrometry
- highly efficient
- bone marrow
- simultaneous determination
- molecularly imprinted
- human health