Tandem-trapped ion mobility spectrometry/mass spectrometry coupled with ultraviolet photodissociation.
Fanny C LiuMark E RidgewayJ S Raaj Vellore WinfredNicolas C PolferJusung LeeAlina TheisenChristopher A WoottonMelvin A ParkChristian BleiholderPublished in: Rapid communications in mass spectrometry : RCM (2021)
The data demonstrate that UVPD carried out at elevated pressures of 2-3 mbar yields extensive fragment ions rich in information about the protein and that their exhaustive analysis requires IMS separation post-UVPD. Therefore, because UVPD and tTIMS/MS each have been shown to be valuable techniques on their own merit in proteomics, our contribution here underscores the potential of combining tTIMS/MS with UVPD for structural proteomics.
Keyphrases
- mass spectrometry
- liquid chromatography
- gas chromatography
- high resolution
- high performance liquid chromatography
- capillary electrophoresis
- tandem mass spectrometry
- solid phase extraction
- electronic health record
- quantum dots
- health information
- social media
- machine learning
- binding protein
- data analysis
- water soluble