Conjugated anionic PEG-citrate G2 dendrimer with multi-epitopic HIV-1 vaccine candidate enhance the cellular immune responses in mice.
Asghar AbdoliNina RadmehrAzam BolhassaniAkram EidiParvaneh MehrbodFatemeh MotevalliZahra KianmehrMohsen ChianiMehdi MahdaviShaghayegh YazdaniMehdi Shafiee ArdestaniMohammad Reza KandiMohammad Reza AghasadeghiPublished in: Artificial cells, nanomedicine, and biotechnology (2017)
Multi-epitope vaccines might cause immunity against multiple antigenic targets. Four immunodominant epitopes of HIV-1 genome were used to construct a polytope vaccine, formulated by dendrimer. Two regimens of polytopes mixture with dendrimer were utilized to immunize BALB/c mice. Adjuvants were also used to boost immune responses. The conjugated polytope could arouse significant cellular immune responses (P < 0.05) and Th1 response showed higher intensity compared to Th2 (P < 0.05). Our study depicted that conjugated dendrimer with multi-epitopic rHIVtop4 would efficiently induce cell-mediated immune responses and might be considered as promising delivery system for vaccines formulation.
Keyphrases
- immune response
- antiretroviral therapy
- hiv positive
- hiv infected
- photodynamic therapy
- human immunodeficiency virus
- hiv testing
- dendritic cells
- hepatitis c virus
- toll like receptor
- hiv aids
- drug delivery
- high fat diet induced
- men who have sex with men
- single cell
- gene expression
- south africa
- stem cells
- metabolic syndrome
- genome wide
- high intensity
- insulin resistance
- bone marrow