A single dose intranasal combination panebolavirus vaccine.

A single intranasal vaccination of guinea pigs or ferrets with the trivalent combination vaccine elicited humoral responses to each of the targeted ebolaviruses, including binding and neutralizing antibodies, as well as Fc-mediated effector functions. This vaccine protected animals from death and disease caused by lethal challenges with EBOV, SUDV or BDBV. Notably, the combination vaccine elicited protection which was comparable to that induced by the monovalent vaccines, thus demonstrating the value of this combination trivalent vaccine.