Continuous Live-Cell Culture Imaging and Single-Cell Tracking by Computational Lensfree LED Microscopy.
Gregor ScholzShinta MarianaAgus Budi DharmawanIqbal SyamsuPhilipp HörmannCarsten ReuseJana HartmannKarsten HillerJuan Daniel Prades GarcíaHutomo Suryo WasistoAndreas WaagPublished in: Sensors (Basel, Switzerland) (2019)
Continuous cell culture monitoring as a way of investigating growth, proliferation, and kinetics of biological experiments is in high demand. However, commercially available solutions are typically expensive and large in size. Digital inline-holographic microscopes (DIHM) can provide a cost-effective alternative to conventional microscopes, bridging the gap towards live-cell culture imaging. In this work, a DIHM is built from inexpensive components and applied to different cell cultures. The images are reconstructed by computational methods and the data are analyzed with particle detection and tracking methods. Counting of cells as well as movement tracking of living cells is demonstrated, showing the feasibility of using a field-portable DIHM for basic cell culture investigation and bringing about the potential to deeply understand cell motility.
Keyphrases
- single cell
- living cells
- high resolution
- rna seq
- single molecule
- high throughput
- cell therapy
- fluorescent probe
- optical coherence tomography
- deep learning
- signaling pathway
- electronic health record
- label free
- cell cycle arrest
- escherichia coli
- big data
- convolutional neural network
- stem cells
- machine learning
- risk assessment
- climate change
- fluorescence imaging
- real time pcr
- data analysis
- human health
- loop mediated isothermal amplification