Phospho-RNA sequencing with circAID-p-seq.
Alessia Del PianoTea KecmanMichael SchmidRuggero BarbieriLuciano BrocchieriSilvia TornalettiClaudia FirritoLuca MinatiPaola BernaboIlaria SignoriaFabio LauriaThomas Henry GillingwaterGabriella VieroMassimiliano ClamerPublished in: Nucleic acids research (2021)
Most RNA footprinting approaches that require ribonuclease cleavage generate RNA fragments bearing a phosphate or cyclic phosphate group at their 3' end. Unfortunately, current library preparation protocols rely only on a 3' hydroxyl group for adaptor ligation or poly-A tailing. Here, we developed circAID-p-seq, a PCR-free library preparation for selective 3' phospho-RNA sequencing. As a proof of concept, we applied circAID-p-seq to ribosome profiling, which is based on sequencing of RNA fragments protected by ribosomes after endonuclease digestion. CircAID-p-seq, combined with the dedicated computational pipeline circAidMe, facilitates accurate, fast and highly efficient sequencing of phospho-RNA fragments from eukaryotic cells and tissues. We used circAID-p-seq to portray ribosome occupancy in transcripts, providing a versatile and PCR-free strategy to possibly unravel any endogenous 3'-phospho RNA molecules.