Quantification of Amine- and Alcohol-Containing Metabolites in Saline Samples Using Pre-extraction Benzoyl Chloride Derivatization and Ultrahigh Performance Liquid Chromatography Tandem Mass Spectrometry (UHPLC MS/MS).
Brittany WidnerMelissa C Kido SouleFrank Xavier Ferrer-GonzálezMary Ann MoranElizabeth B KujawinskiPublished in: Analytical chemistry (2021)
Dissolved metabolites serve as nutrition, energy, and chemical signals for microbial systems. However, the full scope and magnitude of these processes in marine systems are unknown, largely due to insufficient methods, including poor extraction of small, polar compounds using common solid-phase extraction resins. Here, we utilized pre-extraction derivatization and ultrahigh performance liquid chromatography electrospray ionization tandem mass spectrometry (UHPLC-ESI-MS/MS) to detect and quantify targeted dissolved metabolites in seawater and saline culture media. Metabolites were derivatized with benzoyl chloride by their primary and secondary amine and alcohol functionalities and quantified using stable isotope-labeled internal standards (SIL-ISs) produced from 13C6-labeled benzoyl chloride. We optimized derivatization, extraction, and sample preparation for field and culture samples and evaluated matrix-derived biases. We have optimized this quantitative method for 73 common metabolites, of which 50 cannot be quantified without derivatization due to low extraction efficiencies. Of the 73 metabolites, 66 were identified in either culture media or seawater and 45 of those were quantified. This derivatization method is sensitive (detection limits = pM to nM), rapid (∼5 min per sample), and high throughput.
Keyphrases
- ms ms
- liquid chromatography tandem mass spectrometry
- tandem mass spectrometry
- solid phase extraction
- ultra high performance liquid chromatography
- liquid chromatography
- high performance liquid chromatography
- simultaneous determination
- molecularly imprinted
- sensitive detection
- mass spectrometry
- high throughput
- gas chromatography
- high resolution mass spectrometry
- gas chromatography mass spectrometry
- high resolution
- quantum dots
- air pollution
- computed tomography
- particulate matter
- microbial community
- ionic liquid
- cancer therapy