Quantitative detection of anti-PLA2R antibodies targeting different epitopes and its clinical application in primary membranous nephropathy.

Yuan QinQingqing WuHuiming ShengTing LiXiaobin LiuXue YangBo LinXiumei ZhouJuan JinLiang WangBiao HuangQiang HeZhigang Hu

Published in: Clinical chemistry and laboratory medicine (2022)

PLA2R domain-specific IgG/IgG4 TRFIAs were established in this study, and detection with anti-PLA2R IgG4 could more sensitively screen the reactivity of patients to the PLA2R domain. Moreover, detection epitope spreading of PLA2R was confirmed which is related to the severity of patients with PMN.

Keyphrases

loop mediated isothermal amplification
end stage renal disease
label free
real time pcr
chronic kidney disease
newly diagnosed
ejection fraction
peritoneal dialysis
cancer therapy
mass spectrometry
patient reported outcomes
single cell