Roar: detecting alternative polyadenylation with standard mRNA sequencing libraries.
Elena GrassiElisa MariellaAntonio LemboIvan MolinerisPaolo ProveroPublished in: BMC bioinformatics (2016)
We implemented the algorithm in a Bioconductor package to facilitate its broad usage in the scientific community. The ability of this approach to detect shortening from libraries with a number of reads comparable to that needed for differential expression analyses makes it useful for investigating if alternative polyadenylation is relevant in a certain biological process without requiring specific experimental assays.