RAFT Synthesis and Characterization of Poly(Butyl- co -2-( N , N -Dimethylamino)Ethyl Acrylates)- block -Poly(Polyethylene Glycol Monomethyl Ether Acrylate) as a Photosensitizer Carrier for Photodynamic Therapy.

Polymer micelles are promising drug delivery systems for highly hydrophobic photosensitizers in photodynamic therapy (PDT) applications. We previously developed pH-responsive polymer micelles consisting of poly(styrene- co -2-( N , N -dimethylamino)ethyl acrylate)- block -poly(polyethylene glycol monomethyl ether acrylate) (P(St- co -DMAEA)- b -PPEGA) for zinc phthalocyanine (ZnPc) delivery. In this study, poly(butyl- co -2-( N , N -dimethylamino)ethyl acrylates)- block -poly(polyethylene glycol monomethyl ether acrylate) (P(BA- co -DMAEA)- b -PPEGA) was synthesized via reversible addition and fragmentation chain transfer (RAFT) polymerization to explore the role of neutral hydrophobic units in photosensitizer delivery. The composition of DMAEA units in P(BA- co -DMAEA) was adjusted to 0.46, which is comparable to that of P(St- co -DMAEA)- b -PPEGA. The size distribution of the P(BA- co -DMAEA)- b -PPEGA micelles changed when the pH decreased from 7.4 to 5.0, indicating their pH-responsive ability. The photosensitizers, 5,10,15,20-tetrakis(pentafluorophenyl)chlorin (TFPC), 5,10,15,20-tetrakis(pentafluorophenyl)porphyrin (TFPP), protoporphyrin IX (PPIX), and ZnPc were examined as payloads for the P(BA- co -DMAEA)- b -PPEGA micelles. The encapsulation efficiency depended on the nature of the photosensitizer. TFPC-loaded P(BA- co -DMAEA)- b -PPEGA micelles exhibited higher photocytotoxicity than free TFPC in the MNNG-induced mutant of the rat murine RGM-1 gastric epithelial cell line (RGK-1), indicating their superiority for photosensitizer delivery. ZnPc-loaded P(BA- co -DMAEA)- b -PPEGA micelles also exhibited superior photocytotoxicity compared to free ZnPc. However, their photocytotoxicity was lower than that of P(St- co -DMAEA)- b -PPEGA. Therefore, neutral hydrophobic units, as well as pH-responsive units, must be designed for the encapsulation of photosensitizers.