Assembly of Plasmonic and Magnetic Nanoparticles with Fluorescent Silica Shell Layer for Tri-functional SERS-Magnetic-Fluorescence Probes and Its Bioapplications.
Hyung-Mo KimDong-Min KimCheolhwan JeongSo Yeon ParkMyeong Geun ChaYuna HaDahye JangSan KyeongXuan-Hung PhamEunil HahmSang Hun LeeDae Hong JeongYoon-Sik LeeDong-Eun KimBong-Hyun JunPublished in: Scientific reports (2018)
In this study, we report on the fabrication of multilayered tri-functional magnetic-SERS-fluorescence nanoprobes (MF-SERS particles) containing clustered superparamagnetic Fe3O4 nanoparticles (NPs), silver NPs, and a fluorescent silica layer. The MF-SERS particles exhibited strong SERS signals from the silver NPs as well as both superparamagnetism and fluorescence. MF-SERS particles were uptaken by cells, allowing successful separation using an external magnetic field. SERS and fluorescence signals could be detected from the NP-containing cells, and CD44 antibody-conjugated MF-SERS particles selectively targeted MDA-MB-231 cells. Based on these properties, MF-SERS particles proved to be a useful nanoprobe for multiplex detection and separation of cancer cells.
Keyphrases
- gold nanoparticles
- sensitive detection
- label free
- raman spectroscopy
- induced apoptosis
- single molecule
- quantum dots
- cell cycle arrest
- energy transfer
- loop mediated isothermal amplification
- endoplasmic reticulum stress
- magnetic nanoparticles
- cell death
- signaling pathway
- photodynamic therapy
- cancer therapy
- fluorescence imaging
- pi k akt
- liquid chromatography
- single cell
- oxidative stress